Ontology highlight
ABSTRACT:
SUBMITTER: Zhang W
PROVIDER: S-EPMC3299411 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Zhang Weiguo W Colmenares Serafin U SU Karpen Gary H GH
Molecular cell 20111229 2
Centromeres are essential chromosomal regions required for kinetochore assembly and chromosome segregation. The composition and organization of centromeric nucleosomes containing the essential histone H3 variant CENP-A (CID in Drosophila) is a fundamental, unresolved issue. Using immunoprecipitation of CID mononucleosomes and cysteine crosslinking, we demonstrate that centromeric nucleosomes contain CID dimers in vivo. Furthermore, CID dimerization and centromeric targeting require a residue imp ...[more]