Unknown

Dataset Information

0

Identification of a new rhoptry neck complex RON9/RON10 in the Apicomplexa parasite Toxoplasma gondii.


ABSTRACT: Apicomplexan parasites secrete and inject into the host cell the content of specialized secretory organelles called rhoptries, which take part into critical processes such as host cell invasion and modulation of the host cell immune response. The rhoptries are structurally and functionally divided into two compartments. The apical duct contains rhoptry neck (RON) proteins that are conserved in Apicomplexa and are involved in formation of the moving junction (MJ) driving parasite invasion. The posterior bulb contains rhoptry proteins (ROPs) unique to an individual genus and, once injected in the host cell act as effector proteins to co-opt host processes and modulate parasite growth and virulence. We describe here two new RON proteins of Toxoplasma gondii, RON9 and RON10, which form a high molecular mass complex. In contrast to the other RONs described to date, this complex was not detected at the MJ during invasion and therefore was not associated to the MJ complex RON2/4/5/8. Disruptions of either RON9 or RON10 gene leads to the retention of the partner in the ER followed by subsequent degradation, suggesting that the RON9/RON10 complex formation is required for proper sorting to the rhoptries. Finally, we show that the absence of RON9/RON10 has no significant impact on the morphology of rhoptry, on the invasion and growth in fibroblasts in vitro or on virulence in vivo. The conservation of RON9 and RON10 in Coccidia and Cryptosporidia suggests a specific relation with development in intestinal epithelial cells.

SUBMITTER: Lamarque MH 

PROVIDER: S-EPMC3299665 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification of a new rhoptry neck complex RON9/RON10 in the Apicomplexa parasite Toxoplasma gondii.

Lamarque Mauld H MH   Papoin Julien J   Finizio Anne-Laure AL   Lentini Gaelle G   Pfaff Alexander W AW   Candolfi Ermanno E   Dubremetz Jean-François JF   Lebrun Maryse M  

PloS one 20120312 3


Apicomplexan parasites secrete and inject into the host cell the content of specialized secretory organelles called rhoptries, which take part into critical processes such as host cell invasion and modulation of the host cell immune response. The rhoptries are structurally and functionally divided into two compartments. The apical duct contains rhoptry neck (RON) proteins that are conserved in Apicomplexa and are involved in formation of the moving junction (MJ) driving parasite invasion. The po  ...[more]

Similar Datasets

| S-EPMC3946946 | biostudies-literature
| S-EPMC6369123 | biostudies-literature
| S-EPMC3824165 | biostudies-other
| S-EPMC2642630 | biostudies-literature
| S-EPMC4930114 | biostudies-literature
| S-EPMC4959664 | biostudies-literature
| S-EPMC4857766 | biostudies-literature
| S-EPMC2532663 | biostudies-literature
| S-EPMC5623912 | biostudies-literature
| S-EPMC5415223 | biostudies-literature