Ontology highlight
ABSTRACT:
SUBMITTER: Montero M
PROVIDER: S-EPMC3302331 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Montero Marinieve M Gulzar Naveed N Klaric Kristina-Ana KA Donald Jason E JE Lepik Christa C Wu Sampson S Tsai Sue S Julien Jean-Philippe JP Hessell Ann J AJ Wang Shixia S Lu Shan S Burton Dennis R DR Pai Emil F EF Degrado William F WF Scott Jamie K JK
Journal of virology 20120111 6
Failure to elicit broadly neutralizing (bNt) antibodies (Abs) against the membrane-proximal external region of HIV-1 gp41 (MPER) reflects the difficulty of mimicking its neutralization-competent structure (NCS). Here, we analyzed MPER antigenicity in the context of the plasma membrane and identified a role for the gp41 transmembrane domain (TM) in exposing the epitopes of three bNt monoclonal Abs (MAbs) (2F5, 4E10, and Z13e1). We transiently expressed DNA constructs encoding gp41 ectodomain frag ...[more]