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Luria-delbruck estimation of turnip mosaic virus mutation rate in vivo.

ABSTRACT: A potential drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs is the ease with which viruses may generate escape mutations. Using a variation of the classic Luria-Delbrück fluctuation assay, we estimated that the spontaneous mutation rate in the artificial microRNA (amiR) target of a plant virus was ca. 6 × 10(-5) per replication event.

SUBMITTER: de la Iglesia F

PROVIDER: S-EPMC3302333 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Luria-delbruck estimation of turnip mosaic virus mutation rate in vivo.

de la Iglesia Francisca F Martínez Fernando F Hillung Julia J Cuevas José M JM Gerrish Philip J PJ Daròs José-Antonio JA Elena Santiago F SF

Journal of virology 20120111 6

A potential drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs is the ease with which viruses may generate escape mutations. Using a variation of the classic Luria-Delbrück fluctuation assay, we estimated that the spontaneous mutation rate in the artificial microRNA (amiR) target of a plant virus was ca. 6 × 10(-5) per replication event. ...[more]

PMID: 22238294

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