Increased furan tolerance in Escherichia coli due to a cryptic ucpA gene.
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ABSTRACT:
SUBMITTER: Wang X
PROVIDER: S-EPMC3302629 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Increased furan tolerance in Escherichia coli due to a cryptic ucpA gene.
Wang Xuan X Miller Elliot N EN Yomano Lorraine P LP Shanmugam K T KT Ingram Lonnie O LO
Applied and environmental microbiology 20120120 7
Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance. ...[more]
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