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Radial sizing of lipid nanotubes using membrane displacement analysis.

ABSTRACT: We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.

SUBMITTER: Stepanyants N

PROVIDER: S-EPMC3303199 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Radial sizing of lipid nanotubes using membrane displacement analysis.

Stepanyants Natalia N Jeffries Gavin D M GD Orwar Owe O Jesorka Aldo A

Nano letters 20120224 3

We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%. ...[more]

PMID: 22313341

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