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Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.

ABSTRACT: By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.

SUBMITTER: Xu K

PROVIDER: S-EPMC3304438 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.

Xu Ke K Babcock Hazen P HP Zhuang Xiaowei X

Nature methods 20120108 2

By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in ...[more]

PMID: 22231642

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