Cytosolic carboxypeptidase 1 is involved in processing ?- and ?-tubulin.

ABSTRACT: The Purkinje cell degeneration (pcd) mouse has a disruption in the gene encoding cytosolic carboxypeptidase 1 (CCP1). This study tested two proposed functions of CCP1: degradation of intracellular peptides and processing of tubulin. Overexpression (2-3-fold) or knockdown (80-90%) of CCP1 in human embryonic kidney 293T cells (HEK293T) did not affect the levels of most intracellular peptides but altered the levels of ?-tubulin lacking two C-terminal amino acids (delta2-tubulin) ? 5-fold, suggesting that tubulin processing is the primary function of CCP1, not peptide degradation. Purified CCP1 produced delta2-tubulin from purified porcine brain ?-tubulin or polymerized HEK293T microtubules. In addition, CCP1 removed Glu residues from the polyglutamyl side chains of porcine brain ?- and ?-tubulin and also generated a form of ?-tubulin with two C-terminal Glu residues removed (delta3-tubulin). Consistent with this, pcd mouse brain showed hyperglutamylation of both ?- and ?-tubulin. The hyperglutamylation of ?- and ?-tubulin and subsequent death of Purkinje cells in pcd mice was counteracted by the knock-out of the gene encoding tubulin tyrosine ligase-like-1, indicating that this enzyme hyperglutamylates ?- and ?-tubulin. Taken together, these results demonstrate a role for CCP1 in the processing of Glu residues from ?- as well as ?-tubulin in vitro and in vivo.

SUBMITTER: Berezniuk I 

PROVIDER: S-EPMC3307270 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature