Ontology highlight
ABSTRACT:
SUBMITTER: Cole NB
PROVIDER: S-EPMC3307366 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Cole Nelson B NB Donaldson Julie G JG
ACS chemical biology 20120113 3
Site-specific labeling of cellular proteins with chemical probes is a powerful tool for live cell imaging of biological processes. One popular system, known as the SNAP-tag, is based on an engineered variant of the 20-kDa DNA repair protein O(6)-alkylguanine-DNA-alkyltransferase (AGT) that covalently reacts with O(6)-benzylguanine (BG) and can be derivatized with a number of reporter groups. For studying the endocytosis and recycling of cell surface proteins, the covalent nature of BG binding to ...[more]