Rapid diagnosis of pandemic (H1N1) 2009 in Cuba.
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ABSTRACT:
SUBMITTER: Acosta B
PROVIDER: S-EPMC3310444 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Publications
Rapid diagnosis of pandemic (H1N1) 2009 in Cuba.
Acosta Belsy B Piñón Alexander A Valdés Odalys O Savón Clara C Arencibía Amely A Guilarte Elías E Grehete Gonzalez G Oropesa Suset S Guelsys Gonzalez G Hernández Bárbara B Goyenechea Angel A Muné Mayra M Kouri Vivian V Guzmán María G MG Llop Alina A
Emerging infectious diseases 20120201 2
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Project description:The 1918 influenza A virus caused the most devastating pandemic, killing approximately 50 million people worldwide. Immunization with 1918-like and classical swine H1N1 virus vaccines results in cross-protective antibodies against the 2009 H1N1 pandemic influenza, indicating antigenic similarities among these viruses. In this study, we demonstrate that vaccination with the 2009 pandemic H1N1 vaccine elicits 1918 virus cross-protective antibodies in mice and humans, and that vaccination or passive transfer of human-positive sera reduced morbidity and conferred full protection from lethal challenge with the 1918 virus in mice. The spread of the 2009 H1N1 influenza virus in the population worldwide, in addition to the large number of individuals already vaccinated, suggests that a large proportion of the population now have cross-protective antibodies against the 1918 virus, greatly alleviating concerns and fears regarding the accidental exposure/release of the 1918 virus from the laboratory and the use of the virus as a bioterrorist agent.
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Project description:During May 2009-April 2010, we analyzed 692 samples of pandemic (H1N1) 2009 virus from patients in Mexico. We detected the H275Y substitution of the neuraminidase gene in a specimen from an infant with pandemic (H1N1) 2009 who was treated with oseltamivir. This virus was susceptible to zanamivir and resistant to adamantanes and oseltamivir.
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