Ontology highlight
ABSTRACT:
SUBMITTER: Barendt PA
PROVIDER: S-EPMC3310771 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Barendt Pamela A PA Shah Najaf A NA Barendt Gregory A GA Sarkar Casim A CA
PLoS genetics 20120322 3
Studies of synthetic, well-defined biomolecular systems can elucidate inherent capabilities that may be difficult to uncover in a native biological context. Here, we used a minimal, reconstituted translation system from Escherichia coli to identify efficient ribosome binding sites (RBSs) in an unbiased, high-throughput manner. We applied ribosome display, a powerful in vitro selection method, to enrich only those mRNA sequences which could direct rapid protein translation. In addition to canonic ...[more]