Glycoproteomic characterization of recombinant mouse ?-dystroglycan.
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ABSTRACT:
SUBMITTER: Harrison R
PROVIDER: S-EPMC3311285 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Publications
Glycoproteomic characterization of recombinant mouse α-dystroglycan.
Harrison Rebecca R Hitchen Paul G PG Panico Maria M Morris Howard R HR Mekhaiel David D Pleass Richard J RJ Dell Anne A Hewitt Jane E JE Haslam Stuart M SM
Glycobiology 20120111 5
α-Dystroglycan (DG) is a key component of the dystrophin-glycoprotein complex. Aberrant glycosylation of the protein has been linked to various forms of congenital muscular dystrophy. Unusually α-DG has previously been demonstrated to be modified with both O-N-acetylgalactosamine and O-mannose initiated glycans. In the present study, Fc-tagged recombinant mouse α-DG was expressed and purified from human embryonic kidney 293T cells. α-DG glycopeptides were characterized by glycoproteomic strategi ...[more]
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