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Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay.

ABSTRACT: BACKGROUND: Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. FINDINGS: A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. CONCLUSIONS: The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.

SUBMITTER: Yang JL

PROVIDER: S-EPMC3312826 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay.

Yang Jin-Long JL Yang Rui R Shen Ke-Fei KF Peng Xiang-Wei XW XIong Tao T Liu Zuo-Hua ZH

Virology journal 20120217

<h4>Background</h4>Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.<h4>Findings</h4>A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a col ...[more]

PMID: 22340141

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