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Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila.

ABSTRACT: Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.

SUBMITTER: Mota AJ

PROVIDER: S-EPMC3313500 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila.

Mota Adolfo Jose AJ Back-Brito Graziella Nuernberg GN Nobrega Francisco G FG

Genetics and molecular biology 20111215 1

Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-speci ...[more]

PMID: 22481884

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Debaryomyces hansenii (Candida famata), a rare human fungal pathogen often misidentified as Pichia guilliermondii (Candida guilliermondii).

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