Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis).
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ABSTRACT:
SUBMITTER: Naskar S
PROVIDER: S-EPMC3313522 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Publications
Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis).
Naskar Soumen S Deb Sitangsu M SM Niranjan Saket K SK Kumar Subodh S Sharma Deepak D Sakaram Durgam D Sharma Arjava A
Genetics and molecular biology 20120101 1
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β(1) domain. Residues associated with important fu ...[more]
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