Ontology highlight
ABSTRACT:
SUBMITTER: Henn A
PROVIDER: S-EPMC33154 | biostudies-literature | 2001 Apr
REPOSITORIES: biostudies-literature
Henn Arnon A Medalia Ohad O Shi Shu-Ping SP Steinberg Michal M Franceschi Francois F Sagi Irit I
Proceedings of the National Academy of Sciences of the United States of America 20010410 9
The Escherichia coli protein DbpA is unique in its subclass of DEAD box RNA helicases, because it possesses ATPase-specific activity toward the peptidyl transferase center in 23S rRNA. Although its remarkable ATPase activity had been well defined toward various substrates, its RNA helicase activity remained to be characterized. Herein, we show by using biochemical assays and atomic force microscopy that DbpA exhibits ATP-stimulated unwinding activity of RNA duplex regardless of its primary seque ...[more]