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GISP: a novel brain-specific protein that promotes surface expression and function of GABA(B) receptors.

ABSTRACT: Synaptic transmission depends on the regulated surface expression of neurotransmitter receptors, but many of the cellular processes required to achieve this remain poorly understood. To better define specific mechanisms for the GABA(B) receptor (GABA(B)R) trafficking, we screened for proteins that bind to the carboxy-terminus of the GABA(B1) subunit. We report the identification and characterization of a novel 130-kDa protein, GPCR interacting scaffolding protein (GISP), that interacts directly with the GABA(B1) subunit via a coiled-coil domain. GISP co-fractionates with GABA(B)R and with the postsynaptic density and co-immunoprecipitates with GABA(B1) and GABA(B2) from rat brain. In cultured hippocampal neurons, GISP displays a punctate dendritic distribution and has an overlapping localization with GABA(B)Rs. When co-expressed with GABA(B)Rs in human embryonic kidney cells, GISP promotes GABA(B)R surface expression and enhances both baclofen-evoked extracellular signal-regulated kinase (ERK) phosphorylation and G-protein inwardly rectifying potassium channel (GIRK) currents. These results suggest that GISP is involved in the forward trafficking and stabilization of functional GABA(B)Rs.

SUBMITTER: Kantamneni S

PROVIDER: S-EPMC3315443 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature

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GISP: a novel brain-specific protein that promotes surface expression and function of GABA(B) receptors.

Kantamneni Sriharsha S Corrêa Sônia A L SA Hodgkinson Gina K GK Meyer Guido G Vinh Ngoc Nga NN Henley Jeremy M JM Nishimune Atsushi A

Journal of neurochemistry 20070104 4

Synaptic transmission depends on the regulated surface expression of neurotransmitter receptors, but many of the cellular processes required to achieve this remain poorly understood. To better define specific mechanisms for the GABA(B) receptor (GABA(B)R) trafficking, we screened for proteins that bind to the carboxy-terminus of the GABA(B1) subunit. We report the identification and characterization of a novel 130-kDa protein, GPCR interacting scaffolding protein (GISP), that interacts directly ...[more]

PMID: 17241134

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Dataset Information

GISP: a novel brain-specific protein that promotes surface expression and function of GABA(B) receptors.

Publications

GISP: a novel brain-specific protein that promotes surface expression and function of GABA(B) receptors.

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