Ontology highlight
ABSTRACT:
SUBMITTER: Steiner FA
PROVIDER: S-EPMC3317158 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Steiner Florian A FA Talbert Paul B PB Kasinathan Sivakanthan S Deal Roger B RB Henikoff Steven S
Genome research 20120104 4
An understanding of developmental processes requires knowledge of transcriptional and epigenetic landscapes at the level of tissues and ultimately individual cells. However, obtaining tissue- or cell-type-specific expression and chromatin profiles for animals has been challenging. Here we describe a method for purifying nuclei from specific cell types of animal models that allows simultaneous determination of both expression and chromatin profiles. The method is based on in vivo biotin-labeling ...[more]