Apatite reduces amelogenin proteolysis by MMP-20 and KLK4 in vitro.
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ABSTRACT:
SUBMITTER: Sun Z
PROVIDER: S-EPMC3318039 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Publications
Apatite reduces amelogenin proteolysis by MMP-20 and KLK4 in vitro.
Sun Z Z Carpiaux W W Fan D D Fan Y Y Lakshminarayanan R R Moradian-Oldak J J
Journal of dental research 20100216 4
Two enamel proteases, matrix metalloproteinase-20 (MMP-20) and kallikrein 4 (KLK4), are known to cleave amelogenin and are necessary for proper enamel formation. However, the effect of hydroxyapatite (HAP) on the proteolytic activity of these enzymes remains unclear. To investigate whether apatite affects normal amelogenin proteolysis, we used 2 different isoforms of amelogenin combined with the appropriate enzymes to analyze proteolytic processing rates in the presence or absence of synthetic h ...[more]
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