ABSTRACT: ?-N-Acetylglucosaminidase (NagZ), encoded by the nagZ gene, is a critical enzyme for basal-level ampC derepression (ampC expression in the absence of ?-lactam challenge) in ampD and dacB mutants of Pseudomonas aeruginosa. Three mutants with a phenotype of basal-level L1 and L2 ?-lactamase derepression in Stenotrophomonas maltophilia have been reported, including KJ?DI (ampD(I) mutant), KJ?mrcA (mrcA mutant), and KJ?DI?mrcA (ampD(I) and mrcA double mutant). In this study, nagZ of S. maltophilia was characterized, and its roles in basal-level ?-lactamase derepression, induced ?-lactamase activities, and ?-lactam resistance of KJ?DI, KJ?mrcA, and KJ?DI?mrcA were evaluated. Expression of the nagZ gene was constitutive and not regulated by AmpR, AmpD(I), AmpN, AmpG, PBP1a, and NagZ. Introduction of ?nagZ into KJ?DI nearly abolished basal-level derepressed ?-lactamase activity; conversely, introduction of ?nagZ into KJ?mrcA did not affect it. At least two activator ligands (ALs) are thus considered responsible for ?-lactamase expression in the S. maltophilia system, specifically, the NagZ-dependent (AL1) and NagZ-independent (AL2) ligands responsible for the basal-level derepressed ?-lactamase activities of KJ?DI and KJ?mrcA, respectively. The contributions of AL1 and AL2 to the induced ?-lactamase activities may vary with the types of ?-lactams. nagZ inactivation did not affect aztreonam-, cefoxitin-, and carbenicillin-induced ?-lactamase activities, but it attenuated cefuroxime- and piperacillin-induced ?-lactamase activities. Introduction of ?nagZ into KJ, KJ?DI, KJ?mrcA, and KJ?DI?mrcA did not significantly change the MICs of the ?-lactams tested except that the MICs of cefuroxime and piperacillin moderately decreased in strains KJ?Z and KJ?DI?Z (nagZ mutants).