Unknown

Dataset Information

0

Discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling.


ABSTRACT: Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from herpes simplex virus 1 (HSV-1) and HSV-2. This is a single-antigen test and has only one diagnostic application. Relatively little is known about HSV antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized. To this end we produced HSV-1 and -2 proteome microarrays in Escherichia coli and probed them against a panel of sera from patients serotyped using commercial gG-1 and gG-2 (gGs for HSV-1 and -2, respectively) enzyme-linked immunosorbent assays. We identified many reactive antigens in both HSV-1 and -2, some of which were type specific (i.e., recognized by HSV-1- or HSV-2-positive donors only) and others of which were nonspecific or cross-reactive (i.e., recognized by both HSV-1- and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli.

SUBMITTER: Kalantari-Dehaghi M 

PROVIDER: S-EPMC3318641 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling.

Kalantari-Dehaghi Mina M   Chun Sookhee S   Chentoufi Aziz Alami AA   Pablo Jozelyn J   Liang Li L   Dasgupta Gargi G   Molina Douglas M DM   Jasinskas Algis A   Nakajima-Sasaki Rie R   Felgner Jiin J   Hermanson Gary G   BenMohamed Lbachir L   Felgner Philip L PL   Davies D Huw DH  

Journal of virology 20120208 8


Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from herpes simplex virus 1 (HSV-1) and HSV-2. This is a single-antigen test and has only one diagnostic application. Relatively little is known about HSV antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized.  ...[more]

Similar Datasets

| S-EPMC3266794 | biostudies-literature
| S-EPMC545576 | biostudies-literature
| S-EPMC7673108 | biostudies-literature
| S-EPMC3599071 | biostudies-literature
| S-EPMC3792640 | biostudies-literature
| S-EPMC3287348 | biostudies-literature
| S-EPMC4524253 | biostudies-literature
| S-EPMC4319894 | biostudies-literature
| S-EPMC7113901 | biostudies-literature
| S-EPMC7481480 | biostudies-literature