Functional cloning and characterization of antibiotic resistance genes from the chicken gut microbiome.
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ABSTRACT:
SUBMITTER: Zhou W
PROVIDER: S-EPMC3318844 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Functional cloning and characterization of antibiotic resistance genes from the chicken gut microbiome.
Zhou Wei W Wang Ying Y Lin Jun J
Applied and environmental microbiology 20120127 8
Culture-independent sampling in conjunction with a functional cloning approach identified diverse antibiotic resistance genes for different classes of antibiotics in gut microbiomes from both conventionally raised and free-range chickens. Many of the genes are phylogenetically distant from known resistance genes. Two unique genes that conferred ampicillin and spectinomycin resistance were also functional in Campylobacter, a distant relative of the Escherichia coli host used to generate the genom ...[more]
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