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Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.

ABSTRACT: This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.

SUBMITTER: Vella SJ

PROVIDER: S-EPMC3320108 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.

Vella Sarah J SJ Beattie Patrick P Cademartiri Rebecca R Laromaine Anna A Martinez Andres W AW Phillips Scott T ST Mirica Katherine A KA Whitesides George M GM

Analytical chemistry 20120302 6

This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assa ...[more]

PMID: 22390675

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Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.

Publications

Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.

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