Unknown

Dataset Information

0

Transcriptional biomarkers and mechanisms of copper-induced olfactory injury in zebrafish.


ABSTRACT: Metals such as copper disrupt olfactory function in fish. Unfortunately, little is understood of the molecular consequences of copper olfactory impairment, thus hindering the development of relevant diagnostic tools of olfactory injury. To address this critical data gap, we analyzed gene expression within olfactory tissues of adult zebrafish exposed to CuCl2 (6, 16, 40 ppb) for 24 h. Transcriptional markers of copper impairment within the entire olfactory system were identified and specific genes of interest (e.g., S100a, parvalbumin 8, olfactory marker protein, and calbindin 2-like protein) were confirmed with quantitative real-time PCR. In addition, we performed gene set analysis (GSA) using both a priori and custom pathways of gene sets specifically targeting the olfactory signal transduction (OST) pathway. These analyses revealed down-regulated gene sets related to calcium channels and ion transport, g-proteins, and olfactory receptors. Collectively, these data demonstrate that copper causes a depression of transcription of key genes within the OST pathway and elsewhere within olfactory tissues, likely resulting in an olfactory system less responsive to odorants. Further, these data provide a mechanistic explanation in support of earlier studies of functional olfactory impairment in fish following copper exposure.

SUBMITTER: Tilton F 

PROVIDER: S-EPMC3321378 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Transcriptional biomarkers and mechanisms of copper-induced olfactory injury in zebrafish.

Tilton Fred F   Tilton Susan C SC   Bammler Theo K TK   Beyer Richard R   Farin Frederico F   Stapleton Patricia L PL   Gallagher Evan P EP  

Environmental science & technology 20081201 24


Metals such as copper disrupt olfactory function in fish. Unfortunately, little is understood of the molecular consequences of copper olfactory impairment, thus hindering the development of relevant diagnostic tools of olfactory injury. To address this critical data gap, we analyzed gene expression within olfactory tissues of adult zebrafish exposed to CuCl2 (6, 16, 40 ppb) for 24 h. Transcriptional markers of copper impairment within the entire olfactory system were identified and specific gene  ...[more]

Similar Datasets

| S-EPMC4750873 | biostudies-literature
2014-04-30 | GSE46891 | GEO
2014-04-30 | E-GEOD-46891 | biostudies-arrayexpress
| S-EPMC6404594 | biostudies-literature
| S-EPMC6944286 | biostudies-literature
| S-EPMC3991301 | biostudies-literature
| S-EPMC2864754 | biostudies-literature
| S-EPMC6309551 | biostudies-literature
| S-EPMC6997376 | biostudies-literature
| S-EPMC8000729 | biostudies-literature