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Morphine and galectin-1 modulate HIV-1 infection of human monocyte-derived macrophages.


ABSTRACT: Morphine is a widely abused, addictive drug that modulates immune function. Macrophages are a primary reservoir of HIV-1; therefore, they play a role in the development of this disease, as well as impact the overall course of disease progression. Galectin-1 is a member of a family of ?-galactoside-binding lectins that are soluble adhesion molecules and that mediate direct cell-pathogen interactions during HIV-1 viral adhesion. Because the drug abuse epidemic and the HIV-1 epidemic are closely interrelated, we propose that increased expression of galectin-1 induced by morphine may modulate HIV-1 infection of human monocyte-derived macrophages (MDMs). In this article, we show that galectin-1 gene and protein expression are potentiated by incubation with morphine. Confirming previous studies, morphine alone or galectin-1 alone enhance HIV-1 infection of MDMs. Concomitant incubation with exogenous galectin-1 and morphine potentiated HIV-1 infection of MDMs. We used a nanotechnology approach that uses gold nanorod-galectin-1 small interfering RNA complexes (nanoplexes) to inhibit gene expression for galectin-1. We found that nanoplexes silenced gene expression for galectin-1, and they reversed the effects of morphine on galectin-1 expression. Furthermore, the effects of morphine on HIV-1 infection were reduced in the presence of the nanoplex.

SUBMITTER: Reynolds JL 

PROVIDER: S-EPMC3324598 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Morphine and galectin-1 modulate HIV-1 infection of human monocyte-derived macrophages.

Reynolds Jessica L JL   Law Wing Cheung WC   Mahajan Supriya D SD   Aalinkeel Ravikumar R   Nair Bindukumar B   Sykes Donald E DE   Mammen Manoj J MJ   Yong Ken-Tye KT   Hui Rui R   Prasad Paras N PN   Schwartz Stanley A SA  

Journal of immunology (Baltimore, Md. : 1950) 20120319 8


Morphine is a widely abused, addictive drug that modulates immune function. Macrophages are a primary reservoir of HIV-1; therefore, they play a role in the development of this disease, as well as impact the overall course of disease progression. Galectin-1 is a member of a family of β-galactoside-binding lectins that are soluble adhesion molecules and that mediate direct cell-pathogen interactions during HIV-1 viral adhesion. Because the drug abuse epidemic and the HIV-1 epidemic are closely in  ...[more]

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