Ontology highlight
ABSTRACT:
SUBMITTER: Cardarelli F
PROVIDER: S-EPMC3325589 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Cardarelli Francesco F Tosti Luca L Serresi Michela M Beltram Fabio F Bizzarri Ranieri R
The Journal of biological chemistry 20111221 8
A quantitative description of carrier-mediated nuclear export in live cells is presented. To this end, we fused a prototypical leucine-rich nuclear export signal (NES) to GFP as a cargo model and expressed the fluorescent chimera in live CHO-K1 cells. By modeling FRAP data, we calculate the NES affinity for the export machinery and the maximum rate of nuclear export achievable at saturation of endogenous carriers. The measured active-export time through the Nuclear Pore Complex (NPC) is 18 ms, r ...[more]