Ontology highlight
ABSTRACT:
SUBMITTER: Core AB
PROVIDER: S-EPMC3325703 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Core Amanda B AB Reyna Arlene E AE Conaway Evan A EA Bradham Cynthia A CA
Proceedings of the National Academy of Sciences of the United States of America 20120319 14
Sea urchins are an important model for experiments at the intersection of development and systems biology, and technical innovations that enhance the utility of this model are of great value. This study explores pantropic retroviruses as a transduction tool for sea urchin embryos, and demonstrates that pantropic retroviruses infect sea urchin embryos with high efficiency and genomically integrate at a copy number of one per cell. We successfully used a self-inactivation strategy to both insert a ...[more]