Ontology highlight
ABSTRACT:
SUBMITTER: Chen Z
PROVIDER: S-EPMC3326271 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Chen Zheng Z Shao Lin L Ye Jing J Li Yongmao Y Huang Shaomei S Chen Huanchun H Cao Shengbo S
Hybridoma (2005) 20120401 2
Non-structural proteins NS3 and NS5 of Japanese encephalitis virus (JEV) were expressed in Escherichia coli and purified by dialysis. Two monoclonal antibodies (MAbs) named 1H7 and 2D4 against NS3 protein and three MAbs named 3C4, 3H7, and 3F10 against NS5 protein were generated by fusing mouse myeloma cell line SP2/0 with spleen lymphocytes from NS3 or NS5 protein immunized mice. Then activity of MAbs was characterized by enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and ind ...[more]