Ontology highlight
ABSTRACT:
SUBMITTER: Kirby TW
PROVIDER: S-EPMC3326329 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Kirby Thomas W TW DeRose Eugene F EF Cavanaugh Nisha A NA Beard William A WA Shock David D DD Mueller Geoffrey A GA Wilson Samuel H SH London Robert E RE
Nucleic acids research 20111214 7
Binding of the catalytic divalent ion to the ternary DNA polymerase β/gapped DNA/dNTP complex is thought to represent the final step in the assembly of the catalytic complex and is consequently a critical determinant of replicative fidelity. We have analyzed the effects of Mg(2+) and Zn(2+) on the conformational activation process based on NMR measurements of [methyl-(13)C]methionine DNA polymerase β. Unexpectedly, both divalent metals were able to produce a template base-dependent conformationa ...[more]