Molecular cloning of large alternative transcripts based on comparative phylogenetic analysis and exploration of an EST database.
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ABSTRACT:
SUBMITTER: Ji J
PROVIDER: S-EPMC3327802 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Publications
Molecular cloning of large alternative transcripts based on comparative phylogenetic analysis and exploration of an EST database.
Analytical biochemistry 20120301 2
In animals, a gene may be 50 kb or over and contain multiple alternative transcripts with sequences that are not experimentally validated. Under these special circumstances, PCR-based cloning may become very difficult. Here a simple cloning strategy is described using the mNLRC5 gene as an example. We performed comparative phylogenetic analysis between murine and human NLR protein families to anchor the translation start codon, searched an EST database with the 3' end of the genomic DNA sequence ...[more]
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