Stability assessment of different chelating moieties used for elemental labeling of bio-molecules.

ABSTRACT: Integrating elemental labeling in quantitative LC-ICP-MS based bio-analysis requires fundamental experiments concerning the stability of complexes during analysis. In a competitive approach complex stability of the chelating moieties 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraaceticacid (DOTA), 1,4,7-triazacyclononane-N,N',N''-triacetic acid (NOTA) and diethylenetriaminepentaacetic dianhydride (DTPA) in combination with 11 different lanthanides was investigated under typical chromatographic conditions. Measurements were carried out via LC-ICP-QMS using a novel mixed mode separation method. The influence of chromatographic separation, pH and temperature on complex stability constants was assessed regarding further applications of multiplexing in bio-analytical assays. The limit of de

SUBMITTER: Kretschy D 

PROVIDER: S-EPMC3330245 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature