Dataset Information

Aromatase and 5?-reductase type 2 mRNA in the green anole forebrain: an investigation of the effects of sex, season and testosterone manipulation.

ABSTRACT: Aromatase and 5?-reductase (5?R) catalyze the synthesis of testosterone (T) metabolites: estradiol and 5?-dihydrotestosterone, respectively. These enzymes are important in controlling sexual behaviors in male and female vertebrates. To investigate factors contributing to their regulation in reptiles, male and female green anole lizards were gonadectomized during the breeding and non-breeding seasons and treated with a T-filled or blank capsule. In situ hybridization was used to examine main effects of and interactions among sex, season, and T on expression of aromatase and one isozyme of 5?R (5?R2) in three brain regions that control reproductive behaviors: the preoptic area, ventromedial nucleus of the amygdala and ventromedial hypothalamus (VMH). Patterns of mRNA generally paralleled previous evaluations of intact animals. Although no main effects of T were detected, interactions were present in the VMH. Specifically, the density of 5?R2 expressing cells was greater in T-treated than control females in this region, regardless of season. Among breeding males, blank-treated males had a denser population of 5?R2 positive cells than T-treated males. Overall, T appears to have less of a role in the regulation of these enzymes than in other vertebrate groups, which is consistent with the primary role of T (rather than its metabolites) in regulation of reproductive behaviors in lizards. However, further investigation of protein and enzyme activity levels are needed before specific conclusions can be drawn.

SUBMITTER: Cohen RE

PROVIDER: S-EPMC3334470 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Aromatase and 5α-reductase type 2 mRNA in the green anole forebrain: an investigation of the effects of sex, season and testosterone manipulation.

Cohen Rachel E RE Wade Juli J

General and comparative endocrinology 20120202 3

Aromatase and 5α-reductase (5αR) catalyze the synthesis of testosterone (T) metabolites: estradiol and 5α-dihydrotestosterone, respectively. These enzymes are important in controlling sexual behaviors in male and female vertebrates. To investigate factors contributing to their regulation in reptiles, male and female green anole lizards were gonadectomized during the breeding and non-breeding seasons and treated with a T-filled or blank capsule. In situ hybridization was used to examine main effe ...[more]

PMID: 22326351

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Dataset Information

Aromatase and 5?-reductase type 2 mRNA in the green anole forebrain: an investigation of the effects of sex, season and testosterone manipulation.

Publications

Aromatase and 5α-reductase type 2 mRNA in the green anole forebrain: an investigation of the effects of sex, season and testosterone manipulation.

Similar Datasets

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