Ontology highlight
ABSTRACT:
SUBMITTER: Brown CD
PROVIDER: S-EPMC3338147 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Brown Christopher D CD Rusek Max S MS Kiessling Laura L LL
Journal of the American Chemical Society 20120410 15
Naturally occurring carbohydrate polymers are ubiquitous. They are assembled by polymerizing glycosyltransferases, which can generate polysaccharide products with repeating sequence patterns. The fidelity of enzymes of this class is unknown. We report a method for testing the fidelity of carbohydrate polymerase pattern deposition: we synthesized fluorosugar donors and used them as chain termination agents. The requisite nucleotide fluorosugars could be produced from a single intermediate using t ...[more]