Ontology highlight
ABSTRACT:
SUBMITTER: Martincova E
PROVIDER: S-EPMC3338651 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Martincová Eva E Voleman Luboš L Najdrová Vladimíra V De Napoli Maximiliano M Eshar Shiri S Gualdron Melisa M Hopp Christine S CS Sanin David E DE Tembo Dumizulu L DL Van Tyne Daria D Walker Dawn D Marcinčiková Michaela M Tachezy Jan J Doležal Pavel P
PloS one 20120427 4
Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar label ...[more]