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Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli.


ABSTRACT: The depleting petroleum reserve, increasingly severe energy crisis, and global climate change are reigniting enthusiasm for seeking sustainable technologies to replace petroleum as a source of fuel and chemicals. In this paper, the efficiency of the MVA pathway on isoprene production has been improved as follows: firstly, in order to increase MVA production, the source of the "upper pathway" which contains HMG-CoA synthase, acetyl-CoA acetyltransferase and HMG-CoA reductase to covert acetyl-CoA into MVA has been changed from Saccharomyces cerevisiae to Enterococcus faecalis; secondly, to further enhance the production of MVA and isoprene, a alanine 110 of the mvaS gene has been mutated to a glycine. The final genetic strain YJM25 containing the optimized MVA pathway and isoprene synthase from Populus alba can accumulate isoprene up to 6.3 g/L after 40 h of fed-batch cultivation.

SUBMITTER: Yang J 

PROVIDER: S-EPMC3338741 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli.

Yang Jianming J   Xian Mo M   Su Sizheng S   Zhao Guang G   Nie Qingjuan Q   Jiang Xinglin X   Zheng Yanning Y   Liu Wei W  

PloS one 20120427 4


The depleting petroleum reserve, increasingly severe energy crisis, and global climate change are reigniting enthusiasm for seeking sustainable technologies to replace petroleum as a source of fuel and chemicals. In this paper, the efficiency of the MVA pathway on isoprene production has been improved as follows: firstly, in order to increase MVA production, the source of the "upper pathway" which contains HMG-CoA synthase, acetyl-CoA acetyltransferase and HMG-CoA reductase to covert acetyl-CoA  ...[more]

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