Ontology highlight
ABSTRACT:
SUBMITTER: Nam J
PROVIDER: S-EPMC3339872 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Nam Jongmin J Davidson Eric H EH
PloS one 20120426 4
Cis-regulatory DNA sequences causally mediate patterns of gene expression, but efficient experimental analysis of these control systems has remained challenging. Here we develop a new version of "barcoded" DNA-tag reporters, "Nanotags" that permit simultaneous quantitative analysis of up to 130 distinct cis-regulatory modules (CRMs). The activities of these reporters are measured in single experiments by the NanoString RNA counting method and other quantitative procedures. We demonstrate the eff ...[more]