Ontology highlight
ABSTRACT:
SUBMITTER: Bheda P
PROVIDER: S-EPMC3341040 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Bheda Poonam P Swatkoski Stephen S Fiedler Katherine L KL Boeke Jef D JD Cotter Robert J RJ Wolberger Cynthia C
Proceedings of the National Academy of Sciences of the United States of America 20120402 16
Although the biological roles of many members of the sirtuin family of lysine deacetylases have been well characterized, a broader understanding of their role in biology is limited by the challenges in identifying new substrates. We present here an in vitro method that combines biotinylation and mass spectrometry (MS) to identify substrates deacetylated by sirtuins. The method permits labeling of deacetylated residues with amine-reactive biotin on the ε-nitrogen of lysine. The biotin can be util ...[more]