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Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements.

ABSTRACT: Nanofluidic sensing elements have been the focus of recent experiments for numerous applications ranging from nucleic acid fragment sizing to single-molecule DNA sequencing. These applications critically rely on high measurement fidelity, and methods to increase resolution are required. Herein, we describe fabrication and testing of a nanochannel device that enhances measurement resolution by performing multiple measurements (>100) on single DNA molecules. The enhanced measurement resolution enabled length discrimination between a mixture of ?-DNA (48.5 kbp) and T7 DNA (39.9 kbp) molecules, which were detected as transient current changes during translocation of the molecules through the nanochannel. As long DNA molecules are difficult to resolve quickly and with high fidelity with conventional electrophoresis, this approach may yield potentially portable, direct electrical sizing of DNA fragments with high sensitivity and resolution.

SUBMITTER: Sen YH

PROVIDER: S-EPMC3341097 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements.

Sen Yi-Heng YH Jain Tarun T Aguilar Carlos A CA Karnik Rohit R

Lab on a chip 20120202 6

Nanofluidic sensing elements have been the focus of recent experiments for numerous applications ranging from nucleic acid fragment sizing to single-molecule DNA sequencing. These applications critically rely on high measurement fidelity, and methods to increase resolution are required. Herein, we describe fabrication and testing of a nanochannel device that enhances measurement resolution by performing multiple measurements (>100) on single DNA molecules. The enhanced measurement resolution ena ...[more]

PMID: 22298224

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