Ontology highlight
ABSTRACT:
SUBMITTER: Guan L
PROVIDER: S-EPMC3341615 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Guan Lirui L van der Heijden Godfried W GW Bortvin Alex A Greenberg Marc M MM
Chembiochem : a European journal of chemical biology 20110801 14
5-Ethynyl-2'-deoxycytidine triphosphate (EdCTP) was synthesized as a probe to be used in conjunction with fluorescent labeling to facilitate the analysis of the in vivo dynamics of DNA-centered processes (DNA replication, repair and cytosine demethylation). Kinetic analysis showed that EdCTP is accepted as a substrate by Klenow exo(-) and DNA polymerase β. Incorporation of 5-ethynyl-2'-deoxycytidine (EdC) into DNA by these enzymes is, at most, modestly less efficient than native dC. EdC-containi ...[more]