Sequencing by hybridization of long targets.
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ABSTRACT:
SUBMITTER: Qin Y
PROVIDER: S-EPMC3344849 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Sequencing by hybridization of long targets.
Qin Yu Y Schneider Tobias M TM Brenner Michael P MP
PloS one 20120504 5
Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error. ...[more]
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