Real-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum.

ABSTRACT: Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for the detection and identification of these pathogens. Real-time PCR based on the amplification of specific sequences is now being used for the identification and quantification of many infective agents. The present work reports real-time PCR protocols for identification of P. chlamydospora and P. aleophilum. Specificity was demonstrated against purified DNA from 60 P. chlamydospora isolates or 61 P. aleophilum is

SUBMITTER: Martin MT 

PROVIDER: S-EPMC3346403 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature