Ontology highlight
ABSTRACT:
SUBMITTER: Ramsey ME
PROVIDER: S-EPMC3346468 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Ramsey Meghan E ME Hackett Kathleen T KT Kotha Chaitra C Dillard Joseph P JP
Applied and environmental microbiology 20120210 9
We have created new complementation constructs for use in Neisseria gonorrhoeae and Neisseria meningitidis. The constructs contain regions of homology with the chromosome and direct the insertion of a gene of interest into the intergenic region between the genes iga and trpB. In order to increase the available options for gene expression in Neisseria, we designed the constructs to contain one of three different promoters. One of the constructs contains the isopropyl-β-d-thiogalactopyranoside-ind ...[more]