Ontology highlight
ABSTRACT:
SUBMITTER: Gouklani H
PROVIDER: S-EPMC3347352 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Gouklani Hamed H Bull Rowena A RA Beyer Claudia C Coulibaly Fasséli F Gowans Eric J EJ Drummer Heidi E HE Netter Hans J HJ White Peter A PA Haqshenas Gholamreza G
Journal of virology 20120215 9
The p7 protein of hepatitis C virus (HCV) is a viroporin that is dispensable for viral genome replication but plays a critical role in virus morphogenesis. In this study, we generated a JFH1-based intergenotypic chimeric genome that encoded a heterologous genotype 1b (GT1b) p7. The parental intergenotypic chimeric genome was nonviable in human hepatoma cells, and infectious chimeric virions were produced only when cells transfected with the chimeric genomes were passaged several times. Sequence ...[more]