Ontology highlight
ABSTRACT:
SUBMITTER: Eshoo MW
PROVIDER: S-EPMC3348129 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Eshoo Mark W MW Crowder Christopher C CC Rebman Alison W AW Rounds Megan A MA Matthews Heather E HE Picuri John M JM Soloski Mark J MJ Ecker David J DJ Schutzer Steven E SE Aucott John N JN
PloS one 20120508 5
Direct molecular tests in blood for early Lyme disease can be insensitive due to low amount of circulating Borrelia burgdorferi DNA. To address this challenge, we have developed a sensitive strategy to both detect and genotype B. burgdorferi directly from whole blood collected during the initial patient visit. This strategy improved sensitivity by employing 1.25 mL of whole blood, a novel pre-enrichment of the entire specimen extract for Borrelia DNA prior to a multi-locus PCR and electrospray i ...[more]