Lipid-based passivation in nanofluidics.
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ABSTRACT:
SUBMITTER: Persson F
PROVIDER: S-EPMC3348678 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Publications
Lipid-based passivation in nanofluidics.
Persson Fredrik F Fritzsche Joachim J Mir Kalim U KU Modesti Mauro M Westerlund Fredrik F Tegenfeldt Jonas O JO
Nano letters 20120406 5
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that ...[more]
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