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Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice.

ABSTRACT: BACKGROUND: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice. RESULTS: We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations. CONCLUSION: Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia.

SUBMITTER: Hofig I

PROVIDER: S-EPMC3349556 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice.

Höfig Ines I Ehrhardt Harald H Jeremias Irmela I

Cell communication and signaling : CCS 20120326 1

<h4>Background</h4>Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice.<h4>Results</h4>We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple ...[more]

PMID: 22448764

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