Ontology highlight
ABSTRACT:
SUBMITTER: Song BP
PROVIDER: S-EPMC3349838 | biostudies-literature | 2012 Mar-Apr
REPOSITORIES: biostudies-literature
Song Benjamin P BP Jain Surbhi S Lin Selena Y SY Chen Quan Q Block Timothy M TM Song Wei W Brenner Dean E DE Su Ying-Hsiu YH
The Journal of molecular diagnostics : JMD 20120116 2
We demonstrated previously that urine contains low-molecular-weight (LMW) (<300 bp), circulation-derived DNA that can be used to detect cancer-specific mutations if a tumor is present. The goal of this study was to develop an assay to detect the colorectal cancer (CRC)-associated, circulation-derived, epigenetic DNA marker hypermethylated vimentin gene (mVIM) in the urine of patients with CRC. An artificial 18-nucleotide DNA sequence was tagged at the 5' end of the primers of the first PCR cycle ...[more]