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Differential p38-dependent signalling in response to cellular stress and mitogenic stimulation in fibroblasts.


ABSTRACT: p38 MAP kinase is known to be activated by cellular stress finally leading to cell cycle arrest or apoptosis. Furthermore, a tumour suppressor role of p38 MAPK has been proposed. In contrast, a requirement of p38 for proliferation has also been described. To clarify this paradox, we investigated stress- and mitogen-induced p38 signalling in the same cell type using fibroblasts. We demonstrate that - in the same cell line - p38 is activated by mitogens or cellular stress, but p38-dependent signalling is different. Exposure to cellular stress, such as anisomycin, leads to a strong and persistent p38 activation independent of GTPases. As a result, MK2 and downstream the transcription factor CREB are phosphorylated. In contrast, mitogenic stimulation results in a weaker and transient p38 activation, which upstream involves small GTPases and is required for cyclin D1 induction. Consequently, the retinoblastoma protein is phosphorylated and allows G1/S transition. Our data suggest a dual role of p38 and indicate that the level and/or duration of p38 activation determines the cellular response, i.e either proliferation or cell cycle arrest.

SUBMITTER: Faust D 

PROVIDER: S-EPMC3352310 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Differential p38-dependent signalling in response to cellular stress and mitogenic stimulation in fibroblasts.

Faust Dagmar D   Schmitt Christina C   Oesch Franz F   Oesch-Bartlomowicz Barbara B   Schreck Ilona I   Weiss Carsten C   Dietrich Cornelia C  

Cell communication and signaling : CCS 20120309


p38 MAP kinase is known to be activated by cellular stress finally leading to cell cycle arrest or apoptosis. Furthermore, a tumour suppressor role of p38 MAPK has been proposed. In contrast, a requirement of p38 for proliferation has also been described. To clarify this paradox, we investigated stress- and mitogen-induced p38 signalling in the same cell type using fibroblasts. We demonstrate that - in the same cell line - p38 is activated by mitogens or cellular stress, but p38-dependent signal  ...[more]

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