Ontology highlight
ABSTRACT:
SUBMITTER: Geiger A
PROVIDER: S-EPMC3353025 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Geiger Anselm A Russo Luigi L Gensch Thomas T Thestrup Thomas T Becker Stefan S Hopfner Karl-Peter KP Griesinger Christian C Witte Gregor G Griesbeck Oliver O
Biophysical journal 20120515 10
Genetically encoded calcium indicators have become instrumental in imaging signaling in complex tissues and neuronal circuits in vivo. Despite their importance, structure-function relationships of these sensors often remain largely uncharacterized due to their artificial and multimodular composition. Here, we describe a combination of protein engineering and kinetic, spectroscopic, and biophysical analysis of the Förster resonance energy transfer (FRET)-based calcium biosensor TN-XXL. Using fluo ...[more]