Ontology highlight
ABSTRACT:
SUBMITTER: Bassett EA
PROVIDER: S-EPMC3353549 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Developmental cell 20120308 4
Centromeres are defined by the presence of chromatin containing the histone H3 variant, CENP-A, whose assembly into nucleosomes requires the chromatin assembly factor HJURP. We find that whereas surface-exposed residues in the CENP-A targeting domain (CATD) are the primary sequence determinants for HJURP recognition, buried CATD residues that generate rigidity with H4 are also required for efficient incorporation into centromeres. HJURP contact points adjacent to the CATD on the CENP-A surface a ...[more]